LOCUS       40924_13965        6076 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Retroviral vector pCX4pur DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Akagi T, Sasai K, Hanafusa H.
  TITLE     Refractory nature of normal human diploid fibroblasts with respect 
            to oncogene-mediated transformation
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 100 (23), 13567-13572 (2003)
  PUBMED    14597713
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Akagi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2002) Tsuyoshi Akagi, Osaka Bioscience Institute, 
            Molecular Oncology; Furuedai 6-2-4, Suita, Osaka 565-0874, Japan 
            (E-mail:takagi@obi.or.jp, Tel:81-6-6872-4834, Fax:81-6-6871-7521)
REFERENCE   3  (bases 1 to 6076)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6076)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2003"; volume: "100"; issue: "23"; pages:
            "13567-13572"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUN-2002) Tsuyoshi Akagi, Osaka Bioscience Institute, Molecular 
            Oncology"; volume: " Furuedai 6-2-4, Suita, Osaka 565-0874, Japan 
            (E-mail:takagi@obi.or.jp, Tel:81-6-6872-4834, Fax"; pages: 
            "81-6-6871-7521"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        88..467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   467..762
                     /label=R-U5 region of MuLV 5' LTR
                     /note="R-U5 region of MuLV 5' LTR"
     misc_feature    826..1183
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1242..1658
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    1668..2042
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     misc_feature    2044..2106
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    2154..2715
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             2718..3314
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     LTR             3371..3962
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(4263..4851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5018..5875)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5876..5980)
                     /label=AmpR promoter
ORIGIN
        1 tcgaggagct tggcccattg catacgttgt atccatatca taatatgtac atttatattg
       61 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
      121 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      181 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      241 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gactatttac
      301 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      361 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      421 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgcta gagaaccatc
      481 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
      541 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
      601 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
      661 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
      721 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
      781 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
      841 ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta
      901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttcggaac
      961 acccggccgc aaccctggga gacgtcccag ggacttcggg ggccgttttt gtggcccgac
     1021 ctgagtccaa aaatcccgat cgttttggac tctttggtgc acccccctaa taggagggat
     1081 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
     1141 tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt
     1201 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
     1261 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1321 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1381 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1441 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1501 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1561 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1621 cctcccttta tccagccctc actccttctc taggcgcccc catatggcca tatgagatct
     1681 tatatggggc acccccgccc cttgtaaact tccctgaccc tgacttgaca agagttacta
     1741 acagcccctc tctccaagct cacttacagg ctctctactt agtccagcac gaagtctgga
     1801 gacctctggc ggcagcctac caagaacaac tggaccgacc ggtggtacct cacccttacc
     1861 gagtcggcga cacagtgtgg gtccgccgac accagactaa gaacctagaa cctcgctgga
     1921 aaggacctta cacagtcctg ctgaccaccc ccaccgccct caaagtagac ggcatcgcag
     1981 cttggataca cgccgcccac gtgaaggctg ccgaccccgg gggtggacca tcctctagac
     2041 tgccggatcc cagtgtggtg gtacgggaat tccggaccga cgcgtgcggc cgcggtaaca
     2101 attgttaact aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc
     2161 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     2221 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     2281 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     2341 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     2401 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     2461 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     2521 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     2581 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     2641 tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     2701 aaaaacacga taataccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     2761 acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     2821 acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     2881 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     2941 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     3001 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     3061 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     3121 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     3181 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     3241 gcttcaccgt caccgccgac gtcgagtgcc cgaaggaccg cgcgacctgg tgcatgaccc
     3301 gcaagcccgg tgcctgacgc ctcgacacga taaaataaaa gattttattt agtctccaga
     3361 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     3421 tttggaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     3481 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     3541 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     3601 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
     3661 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     3721 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     3781 cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact
     3841 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     3901 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     3961 cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattaa cattaaatgg
     4021 ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     4081 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4141 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4201 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4261 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4321 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4381 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4441 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4501 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4561 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4621 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4681 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     4741 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4801 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4861 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4921 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccggc cgcaaatcaa
     4981 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5041 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5101 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5161 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5221 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5281 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5341 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5401 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5461 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5521 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5581 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     5641 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5701 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5761 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5821 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5881 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5941 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6001 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     6061 cgaggccctt tcgtcc
//
